Its wide popularity across disciplines is a testament to the advice and guidance given therein.
This paper, which is actually a follow-up response to criticism of his 2005 paper that started this debate, explains part of the reason for the schizophrenic reporting that’s so common in mainstream health reporting.
help you make true statements, not about human health interventions, but about ecological ones. A map of human genome variation from population-scale sequencing.
The 1000 Genomes Project reported in this paper is an ambitious attempt to take the data acquired in the Human Genome Project and figure out what it means.
RNAseq avoids these problems and makes it possible to sequence with greater diversity and breadth. Tied for second place, this paper reports the synthesis of a bacterial genome entirely from scratch.
Starting from nothing but a digital copy of the sequence and bottle of reagents on the shelf, they made the entire sequence of the DNA which, when transplanted into a cell, gave rise to a entirely different bacterial strain, entirely controlled by the originally- synthesized DNA.
24 of the 34 biological sciences subdisciplines are represented and the axis scales and color schemes remain constant throughout. Given the volume of published research featuring some form of microarray analysis in the past decade, it’s no surprise that this technique, which presents a major advance over the existing technology, would be as widely read as it is.
Data analysis was done in R and graphs were prepared using ggplot2. After much initial enthusiasm, it became clear that microarrays had serious problems holding the technique back from wider application – sequences have to exist to be probed for, the sample prep required to get enough material for detection skewed the relative proportions of the individual sequences, and background noise prevented the detection of rare species. If Snyder’s paper on RNAseq is popular among Mendeley readers, you’d also expect this review of the various types of next-generation sequencing to be very popular as well. Shendure worked with George Church, a hero of the personal genomics and DIY Biology movements, on the technology underlying the open sequencing platform called the Polonator and in this paper he discusses the current start of the art in sequencing technology. Creation of a bacterial cell controlled by a chemically synthesized genome.
In fact, one useful way of looking at it is that nothing is ever proven true.
Rather, the alternative theories are shown to be less and less likely, and that happens through the processes that Ioannidis describes. Quantifying biodiversity: procedures and pitfalls in the measurement and comparison of species richness Another common theme among the top papers is biodiversity, with 3 of the top 10 papers dealing with this subject.