As with many assays and kits, many people who use the luciferase reporter assay don’t take the time to fully understand how it works.
As with many assays and kits, many people who use the luciferase reporter assay don’t take the time to fully understand how it works.Tags: Homework For Elementary StudentsDigital Australia ThesisBusiness Plan For A StartupAnswers To Wileyplus Accounting HomeworkSales Business Plan Template WordEssay On Dowry In Pakistan
Some of the energy released by this reaction is in the form of light.
This reaction is highly energetically efficient, meaning nearly all the energy put into the reaction is rapidly converted to light.
The technique is capable of high sensitivity, with μg/L quantities easily detectable and ng/L possible with specialist assays.
The basic technique consists of placing a fixed quantity of an antibody in a tube together with a fixed quantity of the labelled drug, and the test sample that contains the drug to be assayed.
It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously.
It has broad applications across various fields of cell and molecular biology – wherever you want to measure or track expression of a cloned gene.
All in all, the luciferase reporter assay may be just the thing you need to shed some light on your project from hell!
Immunoassays have a firm place among routine methods for the analysis of drugs in biological fluids and other matrices.