Emit Assay Principle

Emit Assay Principle-73
As with many assays and kits, many people who use the luciferase reporter assay don’t take the time to fully understand how it works.

As with many assays and kits, many people who use the luciferase reporter assay don’t take the time to fully understand how it works.

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Some of the energy released by this reaction is in the form of light.

This reaction is highly energetically efficient, meaning nearly all the energy put into the reaction is rapidly converted to light.

The technique is capable of high sensitivity, with μg/L quantities easily detectable and ng/L possible with specialist assays.

The basic technique consists of placing a fixed quantity of an antibody in a tube together with a fixed quantity of the labelled drug, and the test sample that contains the drug to be assayed.

It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously.

It has broad applications across various fields of cell and molecular biology – wherever you want to measure or track expression of a cloned gene.

All in all, the luciferase reporter assay may be just the thing you need to shed some light on your project from hell!

Immunoassays have a firm place among routine methods for the analysis of drugs in biological fluids and other matrices.

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Comments Emit Assay Principle

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  • Enzyme Multiplied Immunoassay - Boomer
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    Principle of EMIT Redrawn from Pieper and Rutledge, Laboratory Techniques for Pharmacists, Upjohn 1989, page40, figure 12 Procedure • Mix sample containing drug with fixed quantity of enzyme bound drug, and antibody • Add substrate • Measure abs orbance at 15 and 45 seconds after substrate addition • ∆ Absorbance Reaction rate Drug…

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  • The Luciferase Reporter Assay How it works - Bitesize Bio
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    The reaction at the heart of the Luciferase Reporter Assay. The most famous one of these enzymes is the firefly luciferase. Fireflies are able to emit light via a chemical reaction in which luciferin is converted to oxyluciferin by the luciferase enzyme. Some of the energy released by this reaction is in the form of light.…

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